Bacteriological profile and antimicrobial sensitivity pattern in sterile body fluids from a Tertiary Care Teaching Hospital in Solapur, Maharashtra
Dr. Anu Sharma, Dr. NK Shaikh, Dr. Sonal Agarwal
Sterile fluids like CSF (cerebrospinal fluid), pleural fluid, pericardial fluid, peritoneal fluid and synovial fluid can be infected with micro-organisms which can result into severe morbidity and mortality rates amongst patients. The aim of the present study is to look into the bacteriological profile and antibiogram of sterile fluids. Material & Method: Study was conducted for a period of 6 months. All the sterile fluids sent to the Microbiology department for culture & sensitivity were included in the study. Samples were subjected to Gram stain for provisional report and inoculated on culture media and incubated at 370 C for 18-24 hours, identification was done on the basis of standard protocols. Antibiotic sensitivity testing was done as per CLSI guidelines. Results: Out of total of 341 sterile fluids received for culture and sensitivity in Microbiology department, growth was seen in 104(30.50%) samples while 237(70%) samples were sterile. Peritoneal fluid showed growth in 56 (54%) sample followed by pleural fluid 38(36.52%) and CSF 10(9.61%). All other samples were sterile. Most common age group was between 15-59 years- 76(73.07%) followed by age group of more than 60 years 16(15.4%) and minimum cases were reported in 1-14 years 12(11.53%) age group. Present study shows gram negative bacteria n=99(95.2%) were most commonly isolated followed by gram positive bacteria n=5(4.80%). Amongst gram negative isolates Acinetobacter baumannii 29(28%) were most commonly isolated followed by E.coli 27(26%), Pseudomonas aeruginosa 19(18.3), Citrobacter koseri 14(13.5%) and Klebsiella pneumoniae 8(8%). Amongst gram positive bacteria n=5 all the strains were MRSA (4.80%). Antibiotic sensitivity pattern among gram negative bacteria shows multidrug resistance pattern with 58.33% sensitivity to Imipenem followed by Amikacin 42.53%, Cefotaxime 40.3%, and Ceftazidime 22.20%. Among gram positive bacteria all isolates were sensitive to Vancomycin and Linezolid, about 80% sensitivity was seen for Clindamycin, Gentamicin. High resistance was noted for Erythromycin (80%) and Ciprofloxacin (60%).